A kit for measuring the thrombin generation in a sample of a...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/56 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2558928

The invention provides a kit for measuring the thrombin generation in a sample of a patient's blood or plasma, or in a sample of clotting factors. The kit contains lyophilized tissue factor/phospholipid-complex and a lyophilized mixture containing a thrombin-substrate and CaCl2. The invention also provides processes for preparing the reagents for the kit. The kit can be used in a method for measuring the thrombin generation in a sample, wherein it is possible to detect changes in thrombin generation kinetics, for example after administration of inhibitor bypassing agents to a patient who has developed inhibitors to an exogenous clotting factor such as Factor VIII.

L'invention concerne un nécessaire destiné à mesurer la production de thrombine dans un échantillon de sang ou de plasma d'un patient, ou dans un échantillon de facteurs de coagulation. Le nécessaire contient un complexe facteur de tissu lyophilisé/phospholipide et un mélange lyophilisé contenant un substrat de thrombine et du CaCl2. L'invention concerne également des procédés de réactifs destinés à ce nécessaire. Le nécessaire peut être utilisé dans un procédé de mesure de la production de thrombine dans un échantillon dans lequel il est possible de détecter des changements de cinétique de production de thrombine, par exemple la préadministration d'agents de dérivation d'inhibiteurs à un patient qui a développé des inhibiteurs au niveau d'un facteur de coagulation hexogène tel qu'un facteur VIII.

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