A laser illumination system in fluorescent microscopy

G - Physics – 01 – N

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G01N 21/64 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2652027

Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to Time Delay Integration imaging (TDI). Incorporation of TDl technology into image cytometry analysis, like CellTracks®, makes it possible to image moving objects with very high sensitivity and signal-to-noise ratios. Implementation of TDI camera technology with dual excitation and multispectral imaging of enriched.rare cells provides a rapid system for detection, enumeration, differentiation and characterization of imaged rare cells on the basis of size, morphology and immunophenotype.

La présente invention concerne des dispositifs et des procédés pour la collecte et l'analyse automatisées d'images permettant l'identification ou la classification d'objets microscopiques alignés ou déposés sur des surfaces. De tels objets, par exemple des cellules cibles rares marquées, sont immobilisés par voie magnétique ou non magnétique et soumis à une imagerie d'intégration de temporisation (TDI). L'incorporation de la technologie TDI dans l'analyse d'images par cytométrie, telle que CellTracks®, rend possible la formation d'images d'objets en mouvement avec une sensibilité et des rapports signal/bruit très élevés. La mise en oeuvre de la technologie de caméra TDI à double excitation et l'imagerie multispectrale de cellules rares enrichies fournit un système rapide de détection, de dénombrement, de différenciation et de caractérisation de cellules rares en images en fonction de la taille, de la morphologie et de l'immunophénotype.

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