C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 15/66 (2006.01)
Patent
CA 2474172
The present invention relates to combinatorial gene expression libraries and methods for making these. The library comprises a collection of individual cells, the cells being denoted celll,cell2..., cell, wherein i > 2, each cell comprising at least one concatemer of individual oligonucleotide cassettes, each concatemer comprising a nucleotide sequence of the following formula: (rs2-SP-PR-X-TR-SP-rs1) n wherein rsl and rs2 together denote arestriction site, SP denotes aspacerof at least two bases, X denotes anexpressible nucleotide sequence,PRdenotes a promoter, capable of regulatingthe expression of X in the cell,TRdenotes a terminator, and n 2, and whereinat least one concatemer of celllisdifferent from a concatemer cell2.Such libraries are useful in discovery of novel and/or enhanced metabolic pathways leading to the production of novel compounds for e.g. drug discovery and/or to the production of known compounds in novel quantities or in novel compartments of the cells. The expression libraries in particular are composed of host cells capable of co-ordinated and controllable expression of large numbers of heterologous gene in the host cells. co-ordinated and controllable expression of large numbers of heterologous gene i host cells.
La présente invention concerne des échantillothèques d'expression génique combinatoire et les procédés de réalisation correspondant. De telles échantillothèques conviennent à la découverte de canaux du métabolisme nouveaux et/ou améliorés dans l'optique de la production de nouveaux composés, notamment pour la découverte de médicaments et/ou la production de nouveaux composés dans de nouvelles quantités ou dans de nouveaux compartiments des cellules. Ces échantillothèques d'expression notamment se composent de cellules hôtes capables d'expression coordonnée et régulable de grands nombres de gènes hétérologues dans les cellules hôtes.
Goldsmith Neil
Nielsen Soeren V.s.
Soerensen Alexandra M.p. Santana
Deeth Williams Wall Llp
Evolva Ltd.
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