A method and kit for analyzing a target nucleic acid sequence

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2614042

This invention discloses methods for determining the presence of a target nucleic acid sequence using oligonucleotides that cooperate in a nucleic acid processing reaction to produce a detectable signal. In some embodiments, the methods also facilitate quantification of a target nucleic acid sequence. The present invention further discloses kits that can be used to conduct the methods of the present invention.

L'invention concerne des méthodes pour déterminer la présence d'une séquence d'acide nucléique cible à l'aide l'oligonucléotides qui coopèrent dans une réaction de traitement d'acide nucléique pour produire un signal détectable. Dans certains modes de réalisation de l'invention, ces méthodes permettent également de faciliter la quantification d'une séquence d'acide nucléique cible. L'invention concerne également des trousses pouvant être utilisées pour mettre en oeuvre des méthodes de l'invention.

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