A method and kit for detecting antibiotic resistant bacteria

Details A method and kit for detecting antibiotic resistant bacteria A method and kit for detecting antibiotic resistant bacteria

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2761915

The present invention relates to a method and kit for detecting carbapenemase resistantce genes causing carbapenem resistance in bacteria. The invention provides oligonucleotide primers, which can be used in the detection. The method can be used to detect OXA-48, SME, GIM-1, VIM 1-22, SPM, GES 1-10, KPC 1-7, IMI 5 1-3/NMC-A, IMP 1-24 within a single reaction and OXA-23 group, -OXA-24 group, OXA-51 group with ISabal promoter, OXA-55, OXA-58, OXA-60, OXA-62, CMY 1,-10,-11 SFC-1, NDM-1, and SIM-1 within another single reaction.

Cette invention concerne un procédé et un kit pour détecter des gènes de résistance à la carbapénèmase qui induisent une résistance au carbapenem chez les bactéries. L'invention fournit des amorces oligonucléotidiques qui peuvent être utilisées pour la détection. Le procédé peut être utilisé pour détecter OXA-48, SME, GIM-1, VIM 1-22, SPM, GES 1-10, KPC 1-7, IMI 5 1-3/NMC-A, IMP 1-24 lors d'une seule réaction et le groupe OXA-23, le groupe OXA-24, le groupe OXA-51 avec le promoteur ISabal, OXA-55, OXA-58, OXA-60, OXA-62, CMY 1,-10,-11 SFC-1, NDM-1, et SIM-1 lors d'une autre réaction unique.

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