A method and kit for evaluating the metastatic tendency of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07K 14/705 (2006.01) G01N 33/566 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2229108

The present invention concerns a method for evaluating the metastatic tendency of tumor cells by determining the level of expression (mRNA level) of the gene coding for the thrombin receptor (ThR) or by determining the level of the thrombin receptor present on the membranes or within the tumor cells. A high level of either of the above indicates a high metastatic tendency, a low level indicates a low metastatic tendency and an intermediate level indicates a moderate metastatic tendency. The present invention further concerns a kit for use in the above method.

La présente invention concerne un procédé d'évaluation de la tendance métastatique des cellules tumorales qui consiste à évaluer le degré d'expression (taux d'ARNm) du gène codant le récepteur de thrombine (ThR) ou à évaluer la concentration en récepteur de thrombine présent sur les membranes ou à l'intérieur des cellules tumorales. Un degré d'expression élevé du gène codant le récepteur de thrombine indique une forte tendance métastatique, un faible degré d'expression ou une faible concentration indique une tendance métastatique faible et un degré d'expression intermédiaire ou une concentration intermédiaire indique une tendance métastatique intermédiaire. La présente invention concerne en outre un kit permettant de mettre en oeuvre le procédé ci-dessus.

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