C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/705 (2006.01) A61B 17/43 (2006.01) A61D 19/02 (2006.01) A61K 38/17 (2006.01) G01N 33/566 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2242347
A purified polypeptide which provides for initial binding of sperm to oocyte investments and has an active amino acid sequence of SEQ ID NO:12 (Cys-Gln-Ser-Leu-Gln-Glu-Tyr-Leu-Ala-Glu-Gln-Asn-Gln- Arg-Gln-Leu-Glu-Ser-Asn-Lys-Ile-Pro-Glu- Val-Asp-Leu-Ala-Arg-Val-Val-Ala-Pro-Phe-Met-Ser-Asn-Ile-Pro-Leu-Leu-Leu-Tyr- Pro-Gln-Asp-Arg-Pro-Arg-Ser-Gln-Pro-Gln-Pro- Lys-Ala-Asn-Glu-Asp-Val-Cys); or SEQ ID NO:13 (Cys-Glu-Ser-Leu-Gln-Lys-His-Leu- Ala-Glu-Leu-Asn-His-Gln-Lys-Gln-Leu-Glu- Ser-Asn-Lys-Ile-Pro-Glu-Leu-Asp-Met-Thr-Glu-Val-Val-Ala-Pro-Phe-Met-Ala-Asn- Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Gly-Pro- Arg-Ser-Lys-Pro-Gln-Pro-Lys-Asp-Asn-Gly-Asp-Val-Cys); or the shorter but biologically active SEQ ID NO:1 and SEQ ID NO:9 (Tyr-Pro-Gln-Asp-Arg-X-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn, where X is Thr or Pro). The polypeptide is useful in improving sperm-egg binding by fresh sperm, restoring sperm-egg binding following a cryopreservation cycle, enhancing fertilizing potential, and producing antibodies to determine fertilizing potential of sperm, determining sperm-binding sites on egg investments, and contraception.
La présente invention concerne un polypeptide purifié assurant la liaison initiale du sperme sur l'enveloppe de l'ovocyte. Ce polypeptide comporte une séquence d'acide aminé active, la SEQ ID NO:12 (Cys-Gln-Ser-Leu-Gln-Glu-Tyr-Leu-Ala-Glu-Gln-Asn-Gln-Arg-Gln-Leu-Glu-Ser-Asn-Lys-Ile-Pro-Glu-Val-Asp-Leu-Ala-Arg-Val-Val-Ala-Pro-Phe-Met-Ser-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Arg-Pro-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn-Glu-Asp-Val-Cys), ou la SEQ ID NO:13 (Cys-Glu-Ser-Leu-Gln-Lys-His-Leu-Ala-Glu-Leu-Asn-His-Gln-Lys-Gln-Leu-Glu-Ser-Asn-Lys-Ile-Pro-Glu-Leu-Asp-Met-Thr-Glu-Val-Val-Ala-Pro-Phe-Met-Ala-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Gly-Pro-Arg-Ser-Lys-Pro-Gln-Pro-Lys-Asp-Asn-Gly-Asp-Val-Cys), ou, plus courte, mais biologiquement active, la SEQ ID NO:1 et la SEQ ID NO:9 (Tyr-Pro-Gln-Asp-Arg-X-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn, où X est Thr ou Pro). Ce polypeptide convient particulièrement à l'amélioration de la liaison sperme-oeuf par du sperme frais, au rétablissement de la liaison sperme-oeuf à la suite d'un cycle de conservation cryogénique, à l'accroissement du potentiel de fertilisation, ainsi qu'à la production d'anticorps permettant de déterminer le potentiel de fertilisation du sperme, de déterminer les sites de liaison du sperme sur les enveloppes d'oeufs, et à la contraception.
Barbato Guy F.
Cramer Palmer G.
Hammerstedt Roy H.
Dimock Stratton Llp
The Penn State Research Foundation
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