A method for chromatographic removal of prions

Details A method for chromatographic removal of prions A method for chromatographic removal of prions

C - Chemistry, Metallurgy

07

K

C07K 14/805 (2006.01) A61K 38/42 (2006.01) C07K 1/18 (2006.01)

Patent

CA 2259632

A method for removing a prion from a solution comprising the prion and at least one additional biomolecule, comprising directing the solution through an anion-exchange chromatography column under conditions that cause a gradient elution, whereby the prion is separated from at least one of the biomolecules, thereby causing said biomolecule to be collected in an eluate fraction that is distinct from an eluate fraction that includes the prion. In one embodiment, the gradient is a pH gradient, for example, a step gradient. The prion can be a causal agent for a spongiform encephalopathy, such as Creutzfeldt-Jakob disease, Gerstmann-Straussler-Scheinken syndrome, scrapie, or bovine spongiform encephalopathy.

Méthode permettant l'extraction d'un prion d'une solution comprenant le prion et au moins une biomolécule supplémentaire. La méthode consiste à diriger la solution à travers une colonne de chromatographie par échange d'anions dans des conditions provoquant un gradient d'élution, le prion étant séparé d'au moins une des biomolécules, ce qui entraîne le recueil de ladite biomolécule dans une fraction éluée, distincte de la fraction éluée contenant le prion. Selon un mode de réalisation, le gradient est un gradient de pH, par exemple un gradient discontinu. Le prion peut être un agent pathogène de l'encéphalopathie spongiforme, tel que celui de la maladie de Creutzfeld-Jacob, du syndrome Gerstmann-Straussler-Scheinken, de la tremblante du mouton ou de l'encéphalopathie spongiforme bovine.

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