A method for combined sequential agent delivery and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 13/00 (2006.01) A61N 1/30 (2006.01) A61N 1/32 (2006.01) C12N 15/87 (2006.01) G01N 33/487 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2468511

Disclosed is a method for sequential delivery of agents to and/or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and/or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.

L'invention concerne un procédé servant à effectuer l'administration séquentielle d'agents dans une structure cellulaire, ce qui consiste à mettre en application un tube rempli d'électrolyte avec une contre-électrode, à relier ce tube à un générateur de tension ou de courant, à introduire au moins deux agents selon un mode discret dans la solution électrolytique contenue dans le tube placé à proximité de la structure cellulaire, un agent à la fois étant transporté à travers ce tube vers et/ou vers l'intérieur de ladite structure cellulaire dans laquelle un pore a été créé par application d'un champ électrique concentré sur cette structure cellulaire, ce qui permet d'effectuer l'électroporation de ladite structure. Elle concerne également différentes applications de ce procédé, telles que, par exemple, le transfert de solutés imperméables, par exemple, des médicaments ou des gènes vers l'intérieur de la structure cellulaire ou vers l'extérieur de ladite structure.

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