A method for correcting a spectral image for optical...

A - Human Necessities – 61 – B

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A61B 5/00 (2006.01) G01N 21/62 (2006.01)

Patent

CA 2664133

A spectral image is corrected for optical aberrations. Tissue is exposed to a high- intensity, narrow band of light. The narrow band of light is scattered by at least one analyte in the tissue. Raman signals are optically collected from the scattered light. The Raman signals are directed to a wavelength-separating device. The Raman signals are detected as a function of intensity and wavelength to create the spectral image. The spectral image is corrected for optical aberrations using a software algorithm to spatially reassign intensity. The software may be adapted to use a reference image to make dynamic corrections. Fluorescence signals may also be collected.

L'invention concerne Une image spectrale est corrigée pour des opérations otiques. Un tissu est exposé à une lumière haute densité, en bande étroite. La bande étroite de lumière est diffusée par au moins un analyte dans le tissu. Des signaux Raman sont recueillis de manière optique à partir de la lumière diffusée. Les signaux Raman sont dirigés vers un dispositif de séparation de longueur d'onde. Les signaux Raman sont détectés en tant que fonction de l'intensité de longueur d'onde pour créer une image spectrale. L'image spectrale est corrigée pour des aberrations optiques au moyen d'un algorithme logiciel pour réattribuer l'intensité de manière spatiale. Le logiciel peut être adapté pour utiliser une image de référence et effectuer des corrections dynamiques. Des signaux de fluorescence peuvent également être recueillis.

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