A method for delivering a human chorionic gonadotropin (hcg)...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) A61K 9/00 (2006.01) A61K 9/16 (2006.01) A61K 39/39 (2006.01) A61K 47/02 (2006.01) C07K 14/59 (2006.01)

Patent

CA 2650580

This invention comprises a method of immunization in which human chorionic gonadotropin (hCG) vaccine antigens are incorporated into an inorganic salt/biopolymer complex using a simple manufacturing process. The resulting solid matrix is administered to human subjects in the form of microparticles. The method comprises suspending microparticles in an emulsion of a natural oil and water containing an adjuvant compound acceptable for human use and injecting a pharmaceutical dose of the suspension intramuscularly. The vaccine antigens are conjugates of peptide fragments of the beta subunit of hCG with a carrier protein, diphtheria toxoid. Vaccine antigens delivered in this formulation are effective for eliciting antibodies in recipients for the treatment of cancer or hormone-related diseases.

La présente invention concerne un procédé d'immunisation dans lequel des antigènes d'un vaccin à gonadotrophine chorionique humaine (human chorionic gonadotropin; hCG) sont incorporés dans un complexe sel inorganique/biopolymère en utilisant un procédé de fabrication simple. La matrice solide résultante est administrée à des sujets humains sous forme de microparticules. Le procédé comprend la mise en suspension des microparticules dans une émulsion d'huile naturelle et d'eau contenant un composé adjuvant acceptable pour une utilisation humaine et l'injection d'une dose pharmaceutique de la suspension par voie intramusculaire. Les antigènes de vaccin sont des conjugués de fragments peptidiques de la sous-unité bêta de la hCG avec une protéine porteuse, l'anatoxine diphtérique. Les antigènes de vaccin délivrés dans cette formulation provoquent chez les receveurs une production efficace d'anticorps qui permettent de traiter le cancer ou des maladies à cause hormonale.

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