A method for detecting igf1r/chr 15 circulating tumor cells...

Details A method for detecting igf1r/chr 15 circulating tumor cells... A method for detecting igf1r/chr 15 circulating tumor cells...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2717975

The present invention describes methods and probe composition for an automated FISH assay of a blood sample containing circulating tumor cells expressing the IGF-IR gene. The assay provides genetic analysis of suspect circulating tumor cells that have been identified after immunomagnetic selection and fluorescent labeling. Using unique, repeat-free probes to the IGF-IR locus and a chromosome 15 reference probe, cell lines expressing an aberrant number of IGF-IR and Chr 15 signals were detected, including one cell line with a low level of IGF-IR amplification. The ability to directly examine the genetic profile of IGF-IR on circulating tumor cells may provide an automated means for assessing disease and patient response to therapy.

Cette invention concerne des procédés et une composition de sonde pour un dosage automatisé par FISH d'un échantillon sanguin contenant des cellules tumorales circulantes exprimant le gène IGF-IR. Le dosage fournit l'analyse génétique des cellules tumorales circulantes suspectes qui ont été identifiées après sélection immunomagnétique et marquage fluorescent. Au moyen de sondes uniques, sans répétitions, dirigées contre le locus IGF-IR et une sonde de référence chromosome 15, des lignées cellulaires exprimant un nombre aberrant de signaux IGF-IR et Chr 15 ont été détectées, comprenant une lignée cellulaire à bas degré d'amplification IGF-IR. La possibilité d'examiner directement le profil génétique IGF-IR sur des cellules tumorales circulantes offre un moyen automatisé pour évaluer la maladie et la réponse du patient à la thérapie.

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