A method for detecting the binding between mdm2 and the...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/25 (2006.01) A61K 38/10 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2683071

This invention provides both nucleic acid and amino acid sequences encoding for isolated protein-HDM2 binding sites as well as for isolated protein-proteasome binding sites (the ED(X)Y sequences). In a further aspect this invention also provides related nucleic acids, amino acids, vectors, host cells, pharmaceutical compositions and articles of manufacture. This invention further provides methods for determining whether a test compound interacts with the binding between HDM2 and the proteasome, as well as pharmaceutical compositions comprising said test compound, in particular as anti-cancer agent, even more particular for treating cell proliferative disorders in a subject.

L'invention porte sur à la fois sur des séquences d' acides nucléiques et d'acides aminés codant pour les sites de liaison de protéines isolées-HDM2 et sur les sites de liaison de protéines isolées-protéasome (séquence ED(X)Y). L'invention porte également sur des acides nucléiques, des acides aminés, des vecteurs, des cellules hôtes, des préparations pharmaceutiques, et des articles manufacturés, associés. L'invention porte en outre sur des méthodes déterminant si un composé de test interagit avec la liaison HDM2-protéasome, et sur des préparations pharmaceutiques comprenant ledit composé de test, en particulier en tant qu'agent anti-cancéreux ou plus particulièrement pour traiter les troubles de la prolifération cellulaire.

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