A method for detection of analyte in a sample by generating...

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G01N 33/542 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2318170

A process for detecting an analyte which process comprises (a) contacting a sample suspected of containing said analyte with a containment means comprising a barrier which separates signal generating reagents from said sample, in the presence of an element which interacts specifically with said analyte, under conditions whereby interaction between the analyte and the said element results in activation of the signal generating reagents within the containment means on the side of the barrier opposite to the sample, and (b) detecting any signal generated and retained within the containment means from the sample side of the barrier. The process of the invention provides for sensitive detection of very small numbers of analyte materials using measurement techniques which include counting methods such as flow cytometry.

Ce procédé de détection d'un analyte comprend les étapes consistant: (a) à mettre en contact un échantillon sensé contenir ledit analyse avec des moyens de confinement comprenant une barrière séparant des réactifs producteurs de signaux et l'échantillon, en présence d'un élément qui interagit de manière spécifique avec ledit analyte, dans des conditions où l'interaction entre l'analyte et ledit élément se traduit par l'activation des réactifs producteurs de signaux situés dans les moyens de confinement, du côté de la barrière opposée à l'échantillon, et (b) à détecter tout signal produit et retenu dans les moyens de confinement, à partir du côté échantillon de la barrière. Le procédé de l'invention permet une détection sensible de très petits nombres d'analytes à l'aide de techniques de mesure incluant des méthode de comptage comme la cytométrie de flux.

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