A method for detection of colorectal cancer in human samples

G - Physics – 01 – N

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G01N 33/574 (2006.01)

Patent

CA 2526878

The present invention relates to a method of diagnosing colorectal cancer in human samples using several novel protein markers. The markers have been identified by assaying a number of tissue and serum samples from healthy individuals and persons diagnosed with colorectal cancer by means of protein chip technology using mass spectrometry. Differential expression pattern of these markers are indicative of a person having colorectal cancer patient. The diagnosis is based on comparing at least one intensity value, obtained using the method, to a reference value.

La présente invention porte sur une méthode de diagnostic du cancer colorectal dans des échantillons prélevés chez l'homme, cette méthode utilisant plusieurs nouveaux marqueurs de protéines. Les marqueurs ont été identifiés par analyse d'un nombre d'échantillons tissulaires et sérologiques sur des individus en bonne santé et sur des personnes chez lesquelles on a diagnostiqué un cancer colorectal par la technologie des microplaquettes de protéines utilisant la spectrométrie de masse. Le modèle d'expression différentielle de ces marqueurs indique que la personne a un cancer colorectal et/ou prédit le stade de la maladie chez un patient ayant un cancer colorectal. Le diagnostic est basé sur la comparaison d'au moins une valeur d'intensité, obtenue par ce procédé, avec une valeur de référence.

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