A method for determining the antimicrobial agent sensitivity...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/18 (2006.01) C12M 1/16 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2237167

A method for determining the sensitivity of at least one nonparaffinophilic microorganism from a specimen obtained from a patient to different antimicrobial agents, and predetermined quantities thereof. The method includes providing at least one receptacle containing an aqueous solution and adjusting the solution to mimic the in vivo clinical conditions of said patient. The method further includes inoculating the solution with the specimen and placing into the receptacle (i) a slide coated with a carbon source and (ii) a predetermined quantity of an antimicrobial agent to be tested. By observing the nonparaffinophilic microorganism growth or lack thereof on the slide, it can be determined whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the nonparaffinophilic microorganism on the slide. An associated apparatus is also disclosed.

Cette invention concerne un procédé permettant de déterminer, à partir d'un spécimen prélevé chez un patient, la sensibilité d'un ou de plusieurs micro-organismes non paraffinophiles à différents agents anti-microbiens et à des quantités prédéterminées de ces derniers. Ce procédé consiste à mettre une solution aqueuse dans un ou plusieurs réceptacles, et à ajuster cette solution de manière à imiter les conditions cliniques in vivo du patient. Ce procédé consiste ensuite à inoculer le spécimen dans la solution, puis à placer dans le réceptacle (i) une microplaquette recouverte d'une source de carbone, ainsi qu'une quantité prédéterminée (ii) d'un agent anti-microbien à tester. On observe ensuite la croissance de micro-organismes non paraffinophiles ou l'absence de ces derniers sur la microplaquette, ceci afin de déterminer si la quantité prédéterminée d'agent anti-microbien est efficace et permet d'inhiber la croissance des micro-organismes non paraffinophiles sur ladite microplaquette. Cette invention concerne également un appareil associé à ce procédé.

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