A method for determining the antimicrobial agent sensitivity...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/18 (2006.01) C12M 1/16 (2006.01) C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) C12Q 1/24 (2006.01)

Patent

CA 2229059

A method for determining the sensitivity of at least one nonparaffinophilic microorganism from a specimen obtained from a patient to different anticrobial agent and predetermined quantities thereof. The method includes providing at least one receptacle containing an aqueous solution and inoculating the solution with the specimen. The method further includes placing into the receptacle (i) a slide coated with a carbon source and (ii) a predetermined quantity of an antimicrobial agent to be tested. By observing the nonparaffinophilic microorganism growth or lack thereof on the slide, it can be determined whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the nonparaffinophilic microorganism on the slide. An associated apparatus is also disclosed.

La présente invention concerne un procédé de détermination de la sensibilité d'au moins un microorganisme non paraffinophile, provenant d'un échantillon prélevé sur un patient, à différents agents antimicrobiens et à des quantités déterminées de ces agents. Le procédé comprend l'utilisation d'au moins un récipient contenant une solution aqueuse et l'inoculation de l'échantillon à cette solution. Le procédé comprend en outre la mise en place dans le récipient (i) d'une lame enduite d'une source de carbone et (ii) d'une quantité déterminée d'un agent antimicrobien devant être testé. En observant la croissance sur la lame, ou l'absence de croissance, du microorganisme non paraffinophile, on parvient à savoir si la quantité d'agent antimicrobien est efficace pour l'inhibition de la croissance du microorganisme se trouvant sur la lame. L'invention concerne aussi un équipement correspondant.

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