A method for determining the in vivo function of dna coding...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2303327

A method for screening a large number of full or partial cDNA coding sequences to determine which are expressed in a correlated manner is disclosed, as well as a method for determining which coding sequences are responsible for the appearance of a phenotypic trait. Additionally, a method for determining the chromosomal locus controlling the expression of a coding sequence responsible for the appearance of a phenotypic trait is disclosed. Also disclosed is a method for determining the sequential order of a genetic network responsible for the appearance of a phenotypic trait.

L'invention concerne un procédé de criblage d'un grand nombre de séquences de codage d'ADNc intégrales ou partielles, permettant de déterminer celles qui sont exprimées de manière corrélée, ainsi qu'un procédé de détermination des séquences de codage responsables de l'apparition d'une caractéristique phénotypique. Elle porte, de plus, sur un procédé de détermination du locus chromosomique régulant l'expression d'une séquence de codage responsable de l'apparition d'une caractéristique phénotypique. Un procédé de détermination de l'ordre séquentiel d'un réseau génétique responsable de l'apparition d'une caractéristique phénotypique est également décrit.

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