A method for direct nucleic acid sequencing

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2366112

The present invention provides a novel sequencing apparatus and the methods employed to determine the nucleotide sequence of many single nucleic acid molecules simultaneously, in parallel. The methods and apparatus of the present invention offer a rapid, cost effective, high through-put method by which nucleic acid molecules from any source can be readily sequenced without the need for prior amplification of the sample or prior knowledge of any sequence information.

La présente invention concerne un nouvel appareil de séquençage ainsi que des procédés employés pour déterminer la séquence nucléotidique de nombreuses molécules d'acides nucléiques individuelles simultanément, en parallèle. Les procédés et l'appareil de l'invention constituent un procédé rapide, d'un bon rapport coût-efficacité et à haut rendement permettant de séquencer facilement des molécules d'acides nucléiques de toute origine sans nécessiter d'amplification préalable de l'échantillon ou de connaissances préalables de données sur les séquences.

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