C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/00 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2305866
The present invention is drawn to compositions and methods for introducing nucleotide sequences at preferred genomic target sites in a eukaryotic genome. The compositions comprise transfer cassettes which are flanked by nonhomologous recombination sites. The method involves transforming eukaryotic cells containing target sites utilizing non-integrating transformation methods. The method results in efficient integration of nucleotides into predetermined genetic locations and eliminates random DNA integration.
Cette invention concerne des compositions et des procédés utilisés pour introduire des séquences nucléotidiques au niveau de sites cibles génomiques préférés dans un génome d' eucaryote. Les compositions comprennent des cassettes de transfert qui sont flanquées de sites de recombinaison non homologues. Le procédé consiste à transformer des cellules eucaryotes contenant des sites cibles à l'aide du procédé de transformation sans intégration. Le procédé assure l'intégration efficace de nucléotides dans des sites prédéterminés du génome et élimine l'intégration aléatoire d'ADN.
Baszczynski Christopher L.
Gordon-Kamm William J.
Lyznik Leszek Alexander
Pioneer Hi-Bred International Inc.
Smart & Biggar
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