A method for enhancing levels of polyunsaturated fatty acids...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/64 (2006.01) C12N 1/10 (2006.01)

Patent

CA 2519894

The present invention relates to a method of enhancing the levels of Poly- unsaturated fatty acids (PUFA) by 0.5 to 5 times in protests thraustochytrid protests by storing the cells under cold conditions, said method comprising steps of inoculating the protests into a culture medium, growing the culture for 2 to 5 days at room temperature, harvesting the cells by centrifugation to obtain biomass, storing the biomass at about 10~ C for time duration ranging between 12 to 48 hours, and obtaining increased PUFA from the stored biomass.

La présente invention concerne un procédé permettant d'accroître les taux d'acides gras polyinsaturés (PUFA) de 0,5 à 5 fois dans des protistes du type thraustochytrium par stockage des cellules dans des conditions de froid. Ce procédé consiste à inoculer les protistes dans un milieu de culture, à cultiver la culture pendant 2 à 5 jours à température ambiante, à récolter les cellules par centrifugation pour obtenir une biomasse, à stocker cette biomasse à environ 10 ·C pendant une durée comprise entre 12 et 48 heures, puis à obtenir un taux accru de PUFA à partir de cette biomasse stockée.

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