C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/26 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2266807
The present invention provides: A method for evaluating the ability of a compound to inhibit the protoporphyrinogen oxidase activity, which comprises the steps of: (1) culturing a transformant expressing a protoporphyrinogen oxidase gene present in a DNA fragment in a medium containing substantially no protoheme compounds in each comparative system of the presence and absence of a test compound to measure a growth rate of the transformant under each condition, said transformant being resulted from a host cell deficient in the growing ability based on the protoporphyrinogen oxidase activity transformed with the DNA fragment in which a promoter functionable in the host cell and a protoporphyrinogen oxidase gene are operatively linked, and (2) determining the ability of the compound to inhibit the protoporphyrinogen oxidase activity by comparing the growth rates; and the like.
Nishio Shoichi
Shimokawatoko Yasutaka
Marks & Clerk
Sumitomo Chemical Co. Ltd.
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