C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/01 (2006.01) C07K 14/705 (2006.01) C12N 15/79 (2006.01) C12N 15/866 (2006.01) C12P 21/00 (2006.01) C12Q 1/02 (2006.01) G01N 33/566 (2006.01) G01N 33/567 (2006.01)
Patent
CA 2390914
The object of the present invention is to develop a technique for expressing a functional membrane-bound protein by using baculovirus and insect cell expression system. The present invention provides a method for expressing a functional membrane-bound receptor protein which comprises steps of culturing a host infected with at least one type of recombinant baculovirus which contains a gene encoding an interacting protein and a gene encoding a membrane-bound receptor protein which interacts with said interacting protein to perform its function, and co- expressing said interacting protein and said membrane-bound receptor protein in a budded baculovirus released from said host.
Le but de la présente invention est de mettre au point une technique permettant l'expression d'une protéine membranaire fonctionnelle en utilisant des baculovirus et un système d'expression faisant appel à des cellules d'insecte. La présente invention fournit une méthode permettant l'expression d'un récepteur membranaire fonctionnel, laquelle comprend la mise en culture d'un hôte infecté par au moins un type de baculovirus recombinant qui contient un gène codant une protéine d'interaction et un gène codant un récepteur membranaire qui interagit avec ladite protéine d'interaction pour exercer son action, et qui co-exprime ladite protéine d'interaction et ledit récepteur membranaire dans un baculovirus libéré par bourgeonnement dudit hôte.
Hamakubo Takao
Itoh Hiroshi
Kodama Tatsuhiko
Masuda Kazuyuki
Center For Advanced Science And Technology Incubation Ltd.
Dennison Associates
Toudai Tlo Ltd.
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