A method for expressing modified recombinant proteins in a...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/67 (2006.01) A23C 9/20 (2006.01) A61K 38/17 (2006.01) C07K 14/47 (2006.01) C12N 9/12 (2006.01) C12N 15/12 (2006.01) C12N 15/54 (2006.01) C12N 15/62 (2006.01) C12N 15/64 (2006.01) C12N 15/70 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2213857

The present invention provides a method of producing a modified recombinant protein in a host cell using a single vector containing in tandem both the nucleotide sequence encoding an exogenous protein and an enzyme specific for modification of that protein. The invention also specifically provides a method of producing a recombinant phosphorylated human protein in a bacterial system using a single vector containing in tandem the nucleotide sequence encoding the human protein and a human kinase specific for phosphorylalion of that protein.

La présente invention fournit une méthode pour produire une protéine recombinante modifiée dans une cellule hôte à l'aide d'un vecteur unique contenant, en tandem, une séquence nucléotidique codant une protéine exogène et une enzyme spécifique pour la modification de cette protéine. L'invention fournit également de manière spécifique une méthode pour produire une protéine phosphorylée recombinante humaine dans un système bactérien à l'aide d'un vecteur unique contenant, en tandem, la séquence nucléotidique codant la protéine humaine et une kinase humaine spécifique pour la phosphorylation de cette protéine.

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