A method for generating antigen-presenting cells

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0784 (2010.01) A61K 39/00 (2006.01) A61K 39/008 (2006.01) A61P 31/00 (2006.01) A61P 33/02 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2487452

Described is a method for the generation of antigen-presenting cells (APC), preferably bone marrow-derived dendritic cells (BMDC) or peripheral blood- derived dendritic cells, as antigen carrier having immunostimulatory properties for anti-infective treatment comprising the steps of (a) pulsing the APC with antigen and (b) treating the APC with a CpG oligonucleotide. Said APC are useful as an immune prophylactic or immune therapeutic agent against diseases like AIDS, tuberculosis, malaria or leishmaniasis.

Cette invention concerne une méthode de production de cellules présentatrices d'antigène (CPA), de préférence de cellules dendritiques tirées de la moelle osseuse ou de cellules dendritiques tirées du sang périphérique en tant que porteuses d'antigène dotées de propriétés immunostimulatrices pour traitement anti-infectieux. Cette méthode consiste à: (a) pulser les CPA avec un oligonucléotide CpG; et traiter les CPA avec l'olignucléotide CpG. Lesdites CPA sont utiles en tant qu'agents prophylactiques immuns contre des maladies telles que le SIDA, la tuberculose, le paludisme ou la leishmaniose.

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