A method for generating engineered cells for locus specific...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/87 (2006.01) C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2473741

Inhibitors of mismatch repair can be used to generate hypermutable cells and organisms. By inhibiting this process in cells, new cell lines and varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of homologous recombination. These methods are useful for generating targeted loci that can alter the expression profiles of target genes as well as tag exons of gene with a reporter marker to facilitate the monitoring of a given gene product when the host is grown under different conditions or exposed to biological and chemical entities.

Les inhibiteurs de la réparation des appariements peuvent être utilisés pour la génération d'organismes et de cellules hypermutables. Par l'inhibition de ce processus dans les cellules, des nouvelles lignées et variétés cellulaires à propriétés nouvelles et utiles peuvent être préparées plus efficacement que si l'on se repose sur le taux de recombinaison homologue naturel. Lesdites méthodes sont utiles pour la génération de locus ciblés pouvant modifier les profils d'expression de gènes cibles ainsi que d'exons étiquettes d'un gène au moyen d'un marqueur rapporteur pour faciliter le contrôle d'un produit génique donné lorsque l'hôte est cultivé dans différentes conditions ou exposé à des entités biologiques et chimiques.

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