A method for high-density microarray mediated gene...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2386042

The global effect on genes under different environmental conditions can be determined by a comprehensive gene expression profile. The present invention provides a method to monitor the changes in comprehensive cellular gene expression levels at single length resolution by using a high-density microarray prepared with a comprehensive collection of ORFs of a genome. Under different environmental conditions, directly and indirectly affected genes can be detected as the gene expression levels are induced or repressed in comparison to the control.

La présente invention concerne l'établissement d'un profil d'expression de gènes global permettant de déterminer l'effet global de différentes conditions environnementales sur des gènes. La présente invention concerne un procédé permettant d'effectuer le suivi des modifications des taux d'expression de gènes cellulaires globaux à une simple résolution de longueur grâce à l'utilisation d'un jeu ordonné de micro-échantillons haute densité préparé à partir d'un ensemble complet de cadres de lecture ouverte (open reading frame / ORF) d'un génome. Sous différentes conditions environnementales, les gènes directement ou indirectement affectés peuvent être détectés, les taux d'expression des gènes se trouvant augmentés ou diminués par rapport au témoin.

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