C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/40 (2006.01) A01H 1/04 (2006.01) A01H 5/10 (2006.01) C12N 9/26 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2295931
A method for identifying barley with good brewing properties using the thermostability of the barley .beta.-amylase as an indicator. The thermostability of the barley .beta.-amylase significantly affects the degree of the apparent attenuation limit. A method for determining the enzyme activity of an extract solution from one barley seed, an indirect method by an isoelectric point, and an indirect identifying method by DNA polymorphisms of the region containing the .beta.-amylase structural gene have been developed as a method for determining the type of thermostability for a barley .beta.-amylase. The selection method is not affected by environmental or climatic conditions.
Procédé d'identification d'orge possédant de bonnes propriétés brassicoles, sur la base de la thermostabilité de la .beta.-amylase de l'orge en tant qu'indicateur. La thermostabilité de la .beta.-amylase de l'orge influence considérablement la limite d'atténuation apparente. Un procédé permettant de déterminer l'activité enzymatique d'une solution extraite de graine d'orge, un procédé indirect basé sur le point isoélectrique et un procédé d'identification indirecte par polymorphismes de l'ADN de la région contenant le gène structural de .beta.-amylase ont été mis au point en tant que procédé permettant de déterminer le type de thermostabilité d'une .beta.-amylase d'orge. Ledit procédé de sélection n'est pas influencé par des conditions relatives au climat ou à l'environnement.
Fukuda Kensuke
Ito Kazutoshi
Kaneko Takafumi
Kihara Makoto
Sapporo Breweries Ltd.
Smart & Biggar
LandOfFree
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