A method for identifying, expanding, and removing adult stem...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/095 (2010.01) C12N 5/074 (2010.01) C12N 15/113 (2010.01) A01K 67/027 (2006.01) A61K 31/7088 (2006.01) A61K 39/395 (2006.01) C07K 16/28 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) C07K 14/705 (2006.01)

Patent

CA 2696047

The invention relates to the fieldsof biochemistry, pharmacyand oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.

La présente invention concerne les domaines de la biochimie, de la pharmacie et de l'oncologie. L'invention concerne plus particulièrement l'utilisation de nouveaux marqueurs de cellules souches en vue de l'isolement des cellules souches. L'invention concerne en outre les cellules souches obtenues et leur utilisation, par exemple, dans les domaines de la recherche et du traitement, par exemple pour la préparation d'un médicament destiné à traiter les tissus endommagés ou malades. Dans un mode de réalisation, l'invention propose un procédé permettant d'obtenir (ou d'isoler) des cellules souches, consistant éventuellement à préparer une suspension cellulaire à partir d'un tissu ou d'un échantillon d'organe, mettre la suspension cellulaire au contact d'un composé de liaison Lgr 6 ou 5, identifier les cellules liées audit composé de liaison, et éventuellement isoler les cellules souches dudit composé de liaison. L'invention concerne par ailleurs des moyens adaptés au traitement du cancer et, de manière plus spécifique encore, au traitement du cancer par l'éradication des cellules souches cancéreuses.

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