A method for improving plant transformation efficiency by...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01) A01H 4/00 (2006.01) C12N 5/14 (2006.01)

Patent

CA 2538987

It is intended to provide a method of transferring a gene into a plant material via a bacterium belonging to the genus Agrobacterium which comprises: 1) treating the plant material; and 2) infecting the plant material with the agrobacterium; characterized in that, in the above steps 1) and/or 2), use is made of a medium having a metal salt containing copper ion at an elevated concentration. It is also intended to provide a method of constructing a transformant plant characterized by using the gene transfer method as described above.

L'invention concerne un procédé de transfert d'un gène dans une matière végétale par le biais d'une bactérie appartenant au genre Agrobacterium. Ledit procédé consiste (1) à traiter la matière végétale et (2) à l'infecter avec une agrobactérie. Ce procédé est caractérisé en ce qu'aux étapes (1) et (2) susmentionnées, on utilise un milieu contenant un sel métallique qui renferme un ion de cuivre à une concentration élevée. Cette invention a aussi pour objet un procédé d'élaboration d'un végétal de transformant caractérisé par l'utilisation du procédé de transfert génique susmentionné.

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