A method for in vitro molecular evolution of protein function

Details A method for in vitro molecular evolution of protein function A method for in vitro molecular evolution of protein function

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2293819

The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new antibodies or parts thereof having modified characteristics as compared to the parent antibody.

La présente invention concerne un procédé permettant l'évolution in vitro d'une fonction protéique. En particulier, ce procédé se rapporte au réarrangement de segments nucléotidiques obtenus par digestion par exonucléase. Selon la présente invention, il a été montré que les fragments polynucléotidiques dérivés d'une séquence polynucléotidique parent digérée par une exonucléase peuvent être recombinés en vue de générer une séquence polynucléotidique codant un polypeptide présentant les propriétés souhaitées. Ce procédé peut être utilement appliqué à la génération de nouveaux anticorps, ou parties d'anticorps, présentant des propriétés modifiées par rapport à l'anticorps parent.

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