A method for inhibiting tumor invasion or spreading in a...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) A61K 38/00 (2006.01) A61K 38/17 (2006.01) A61K 48/00 (2006.01) C07K 14/705 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2325573

The present invention provides for a method for inhibiting tumor invasion or metastasis in a subject which comprises administering to the subject a therapeutically effective amount of a form of soluble Receptor for Advanced Glycation Endproducts (RAGE). The present invention also provides a method for evaluating the ability of an agent to inhibit tumor invasion in a local cellular environment which comprises: (a) admixing with cell culture media an effective amount of the agent; (b) contacting a tumor cell in cell culture with the media from step (a); (c) determining the amount of spreading of the tumor cell culture, and (d) comparing the amount of spreading of the tumor cell culture determined in step (c) with the amount determined in the absence of the agent, thus evaluating the ability of the agent to inhibit tumor invasion in the local cellular environment. The present invention also provides a pharmaceutical composition which comprises a therapeutically effective amount of the agent evaluated in the aforementioned method and a pharmaceutically acceptable carrier.

L'invention concerne un procédé d'inhibition d'invasion tumorale ou de métastase chez un sujet, qui consiste à administrer au sujet une quantité thérapeutiquement efficace sous la forme d'un récepteur soluble pour produits terminaux de glycation avancée (Receptor for Advanced Glycation Endproducts, RAGE). L'invention concerne également un procédé qui permet d'évaluer la capacité d'un agent à inhiber l'invasion tumorale dans un environnement cellulaire local, comprenant les étapes suivantes : (a) mélange d'une quantité efficace de l'agent avec un milieu de culture cellulaire; (b) contact entre une cellule tumorale en culture cellulaire avec le milieu défini en (a); (c) détermination de l'ampleur de l'extension de la culture cellulaire tumorale, et (d) comparaison entre l'extension mentionnée en (c) et la quantité déterminée en l'absence d'agent. On évalue ainsi l'aptitude de l'agent à inhiber l'invasion tumorale dans l'environnement cellulaire local. L'invention concerne en outre une composition pharmaceutique renfermant une quantité thérapeutiquement efficace de l'agent évalué selon le procédé susmentionné et un vecteur pharmaceutiquement acceptable.

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