A method for introducing substances into cells, and use of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/64 (2006.01) A61K 48/00 (2006.01) C12N 15/87 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2359349

A method for introducing a substance comprising a nucleic acid into a mammalian neural stem cell or progenitor cell, characterized in that said nucleic acid directly interacts with the cell membrane of said cell or a component within said cell membrane whereby the substance comprising said nucleic acid is taken up by the cell via the inherent transport mechanism of the cell, is disclosed. Also different applications of said method are disclosed.

L'invention concerne un procédé d'introduction d'une substance comportant un acide nucléique dans une cellule souche neuronale ou dans un progéniteur de mammifère, caractérisé par le fait que ledit acide nucléique interagit directement avec la membrane cellulaire de ladite cellule ou avec un constituant de ladite membrane cellulaire, la substance comportant ledit acide nucléique étant interceptée par la cellule par l'intermédiaire du mécanisme de transport inhérent à la cellule. Différentes applications dudit procédé sont également décrites.

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