A method for labeled conjugate production

G - Physics – 01 – N

Patent

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G01N 33/532 (2006.01)

Patent

CA 2359234

The present invention relates to methods for coupling labels to particular target moieties. The coupling reactions of the present invention use temporal spacing of the reactants through phase change (i.e. by rapid freezing) to control the initiation and termination of reaction. This process results in a simplified and improved method for linking labels to specific binding moieties using N-hydroxysuccinimide chemistry. The present invention further relates to kits comprising all necessary components to easily and rapidly make protein conjugates.

L'invention concerne des procédés de couplage de marqueurs avec certaines fractions cibles. Les réactions de couplage de l'invention mettent en oeuvre un temps d'espacement entre l'activation des réactifs, par l'intermédiaire d'un changement de phase (par exemple, par congélation rapide), afin de maîtriser le démarrage et la fin de la réaction. Ce procédé se révèle être un moyen simple et perfectionné de liaison de marqueurs avec des fractions de liaison spécifiques, à l'aide d'une chimie à base de N-hydrosuccinimide. L'invention concerne en outre des trousses comprenant tous les composants nécessaires à la préparation facile et rapide de conjugués protéiniques.

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