C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 1/06 (2006.01)
Patent
CA 2192342
A process is disclosed for the large scale isolation and purification of plasmid DNA from large scale microbial fermentations. All three forms of plasmid DNA; supercoil (form I), nicked or relaxed circle (form II), and linearized (form III), are individually isolatable using the disclosed process. Highly purified DNA suitable for inclusion in a pharmaceutical composition is provided by the disclosed process.
L'invention se rapporte à un procédé d'isolation et de purification à grande échelle de l'ADN plasmidique à partir de grandes quantités de fermentations microbiennes. Les trois formes de l'ADN plasmidique, à savoir superhélice (forme I), cercle coupé ou détendu (forme II), et linéarisé (forme III), peuvent être isolées individuellement par le procédé décrit. L'invention se rapporte également à l'ADN hautement purifié approprié pour être incorporé dans une composition pharmaceutique par le procédé décrit.
Lee Ann L.
Sagar Sangeetha
Merck & Co. Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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