A method for monitoring the presence of biofilm-forming...

D - Textiles – Paper – 21 – H

Patent

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D21H 21/04 (2006.01)

Patent

CA 2543586

The invention is directed to a method for detecting the presence of biofilm- forming microorganisms in a paper or board making process for determining the need of an anti-biofilm agent in the process. The method comprises the steps: (a) subjecting a sampler device in the process line for a period of time to enable said microorganisms to form a biofilm in situ in said process on the surface of the sampler, (b) optionally treating said formed biofilm in a solution of a test anti-biofilm agent for a period of time, then (c) contacting said biofilm with a liquid growth medium in a recession of a culturing device for a period of time, and (d) removing the growth solution from the recession of said device and detecting qualitatively and/or quantitatively the presence or absence of a biofilm adhered on the walls of the recession. An assembly kit is also provided.

Cette invention concerne un procédé qui permet de détecter la présence de micro-organismes générateurs de films biologiques dans un processus de fabrication de papier ou de carton, en vue de déterminer la nécessité d'utiliser un agent d'élimination de films biologiques dans le processus. Le procédé consiste à: a) présenter un dispositif d'échantillonnage dans la chaîne de fabrication, pendant un laps de temps, afin de permettre aux micro-organismes de former un film biologique in situ à la surface du dispositif d'échantillonnage, au cours du processus; b) traiter facultativement le film biologique ainsi formé dans une solution d'un agent de mise à l'épreuve de l'agent d'élimination des films biologiques, pendant un laps de temps; c) placer le film biologique au contact d'un milieu de croissance liquide dans un évidement d'un dispositif de mise en culture, pendant un laps de temps; puis d) retirer la solution de croissance de l'évidement du dispositif et détecter qualitativement et/ou quantitativement la présence ou l'absence d'un film biologique collé aux parois de l'évidement. On décrit en outre des nécessaires de montage.

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