A method for obtaining a plant with a genetic lesion in a...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2326835

The present invention provides methods of identifying a plant containing a lesion in a gene sequence flanked in a wild type chromosome by known polynucleotide sequences. The methods comprise providing a collection of nucleic acids from source plants, providing a data base that associates each nucleic acid with its source plant, amplifying the collection of nucleic acids, thereby detecting the lesion, and using the database to identify the source plant carrying the lesion.

L'invention concerne des procédés servant à identifier une plante contenant une lésion dans une séquence de gènes flanquée par des séquences connues de polynucléotides dans un chromosome de type sauvage. Ces procédés consistent à mettre en application un groupe d'acides nucléiques collectés dans des plantes sources, à utiliser une base de données associant chaque acide nucléique à sa plante source, à amplifier ce groupe d'acides nucléiques collectés, ce qui permet de détecter la lésion, et à mettre en oeuvre la base de données afin d'identifier la plante source portant la lésion.

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