C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 9/16 (2006.01) C12N 15/64 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2486900
A quick, simple and efficient in vitro method for generating a random chimaeric and/or mutated gene or genes in a circular polynucleotide molecule is described. The method is based on hybridization of random DNA fragments to a closed, circular DNA template, elongation of the fragments by a DNA polymerase and ligation of the fragments to each other by a DNA ligase resulting in a recombined, closed circular DNA molecule which functions as a new template in a next cycle of chimaerization. The degree of recombination can be controlled by the number of cycle repetitions, the amount of different templates or/and fragments and the ratio of the latter. This procedure is referred to as polymerase chain chimaerization (PCC). The PCC method is useful for generating new improved bio-molecules.
Boenitz-Dulat Mara
Kratzsch Peter
Schmuck Rainer
von Der Eltz Herbert
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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