A method for obtaining plants with reduced susceptibility to...

Details A method for obtaining plants with reduced susceptibility to... A method for obtaining plants with reduced susceptibility to...

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 9/14 (2006.01) C12N 15/82 (2006.01) C12N 15/84 (2006.01)

Patent

CA 2121578

2121578 9310251 PCTABS00022 The present invention provides plants which as a result of the expression of a recombinant DNA have reduced susceptibility to a plant-parasitic nematode. According to a preferred embodiment said recombinant DNA causes disruption of a nematode feeding structure. In another preferred embodiment expression of said recombinant DNA is at least retarding formation of a nematode feeding structure. According to a more preferred embodiment said recombinant DNA comprises: 1) a gene-A which upon expression effects disruption of a nematode feeding structure, said gene-A being placed under the control of a promoter-A that drives expression at least in the nematode feeding structure, and 2) a gene-B which upon expression neutralizes the disruptive effect of gene-A, said gene-B being placed under the control of a promoter-B that drives expression in substantially all of the plant's cells wherein gene-A is expressed, with the proviso that said promoter does not effectively drive expression of gene-B in the nematode feeding structure. A preferred gene-A encodes barnase, while gene-B encodes barstar.

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