A method for preparing lysophosphatidylethanolamine

Details A method for preparing lysophosphatidylethanolamine A method for preparing lysophosphatidylethanolamine

C - Chemistry, Metallurgy

12

P

C12P 13/00 (2006.01)

Patent

CA 2392998

Disclosed herein is a method for purifying the lysophosphatidylethanolamine with high purity by treating the phospholipid mixture with enzymes, followed by solvent fractionaltion without the column purification. The method of the present invention comprises hydrolyzing phospholipid mixture containing 10-99 weight % of phosphatidylethanolamine with phospholipase A2 to produce lysophospholipid; and treating the lysophospholipid with the solvent mixture comprising water and one or more organic solvents selected from the group consisting of low alcohol, hydrocarbon and alkylester to eliminate impurities except lysophosphatidylethanolamine.

La présente invention concerne un procédé de purification de lysophosphatidyléthanolamine avec une grande pureté consistant à traiter le mélange de phospholipides avec des enzymes, puis à le fractionner par solvant sans purification sur colonne. Ce procédé consiste d'abord à hydrolyser un mélange de phospholipides contenant entre 10 et 99 % en poids de phosphatidyléthanolamine avec du phospholipase A2 pour produire du lysophospholipide; puis à traiter ce lysophospholipide avec un mélange de solvants contenant de l'eau et un ou plusieurs solvants organiques sélectionnés dans le groupe constitué par un alcool faible, de l'hydrocarbure et de l'alkylester pour éliminer les impuretés à l'exception du lysophosphatidyléthanolamine.

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