A method for producing l-isoleucine with a fermentation process

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) C12N 15/01 (2006.01) C12P 13/06 (2006.01)

Patent

CA 2177785

The present invention provide a method for the industrial production of L-isoleucine which is useful as pharmaceuticals, foods, feed additives and the like. The method comprises cultivating in a nutrient medium a microorganism belonging to the genus Escherichia which is capable of rapidly growing in a medium containing L- homoserine as the single nitrogen source and has an ability to produce L-isoleucine in the medium, producing and accumulate L-isoleucine in a culture and recovering L- isoleucine therefrom.

La présente invention concerne une méthode pour la production industrielle de L-isoleucine, utile comme produit pharmaceutique, aliment, additif alimentaire ou autre produit analogue. La méthode consiste : à cultiver, dans un milieu nutritif, un microorganisme appartenant au genre Escherichia, qui peut se multiplier rapidement dans un milieu renfermant la L-homosérine comme seule source d'azote, et qui a la capacité de produire la L-isoleucine dans le milieu; à produire et à accumuler la L-isoleucine dans la culture et à récupérer la L- isoleucine à partir de celle-ci.

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