A method for quantitation of collagen in tissue

G - Physics – 01 – N

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G01N 30/86 (2006.01) G01N 33/483 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2664005

For successful wound healing to occur, the newly formed skin must generate tensile strength through collagen deposition. Measurement of collagen in the granulating wound bed may be predictive of successful wound healing. Existing methods for collagen measurement either require specialized equipment, or do not allow for discrimination of potential interfering molecules. Herein described is an accurate, specific and reliable method to extract and quantify collagen from tissue utilizing high pressure liquid chromatography techniques. The method is sensitive enough to measure the small amounts of collagen found in newly healing wounds.

Afin de soigner une blessure avec succès, la peau nouvellement formée doit créer une résistance à la traction grâce à un dépôt de collagène. La mesure du collagène dans le lit de la plaie qui cicatrise peut aider à prévoir le succès de la guérison. Les procédés actuellement disponibles pour mesurer le collagène requièrent un équipement spécialisé ou ne permettent pas de différencier les molécules interférentes potentielles. La présente invention concerne un procédé précis, particulier et fiable qui permet d'extraire et de quantifier le collagène présent dans un tissu à l'aide de techniques de chromatographie liquide à haute performance. Le procédé est suffisamment sensible pour pouvoir mesurer de petites quantités de collagène présent dans les blessures qui viennent d'entamer leur processus de guérison.

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