A method for selective expression of therapeutic genes by...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01)

Patent

CA 2349506

A method for controlling in vivo heat-inducible gene expression for provision of therapeutic polypeptide. The method includes the steps of: (a) providing a construct comprising a polynucleotide encoding a therapeutic polypeptide, wherein the polynucleotide is operatively linked to a heat-inducible promoter; (b) administering the construct to a warm-blooded vertebrate at a site at or near the tumor; and (c) applying heat to the site at or near the tumor to express the therapeutic polypeptide, whereby anti-tumor activity is observed.

Une méthode pour contrôler l'expression de gènes in vivo inductibles par la chaleur pour produire un polypeptide thérapeutique. La méthode comprend les étapes suivantes: (a) développer un construit comprenant un polynucléotide encodant un polypeptide thérapeutique, où le polynucléotide est lié de façon fonctionnelle à un promoteur inductible par la chaleur; (b) administrer le construit à un vertébré à sang chaud sur le site de la tumeur ou à proximité de celle-ci: et appliquer de la chaleur sur le site de la tumeur ou à proximité de celle-ci pour exprimer le polypeptide thérapeutique, un procédé par lequel une activité anti-tumorale est observée.

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