A method for separating, extracting and purifying...

Details A method for separating, extracting and purifying... A method for separating, extracting and purifying...

C - Chemistry, Metallurgy

12

P

C12P 7/62 (2006.01) C08G 63/66 (2006.01)

Patent

CA 2550204

The invention discloses a method for separating, extracting and purifing poly- .beta. -hydroxyalkanoates (PHAs) directly from bacterial fermented broth, which technical field is biotechnological downstream succesive treatment. The method of the invention comprising steps of: physical crushing pretreatment of the bacterial fermented broth; conditioning PH of the bacterial fermented broth to alkalinity, adding anionic surfactant and agitating reaction liquid; separating and extracting precipitation; washing and drying. The process of the invention is simple, cheap, high yield and no pollution, and can realize large scale industrial producting.

Procédé de séparation, d'extraction et de purification de poly- beta -hydroxyalcanoates (PHA) directement à partir d'un milieu de culture bactérien fermenté, par traitements biotechnologiques successifs. Ledit procédé consiste à prétraiter par broyage physique le milieu de culture bactérien fermenté, à ajuster le pH du milieu de culture bactérien fermenté pour qu'il devienne alcalin, à ajouter un tensioactif anionique et à agiter le liquide de réaction, à séparer et extraire le précipité et à opérer une étape de lavage et de séchage. Le procédé selon la présente invention est simple, peu onéreux, produit des hauts rendements, n'entraîne pas de pollution et peut être appliqué pour la production industrielle à grande échelle.

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