A method for simultaneous production of multiple proteins;...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/67 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/79 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2489475

The present invention relates to a method for improving expression of two or more proteins in a (host) cell. The method is suited for production of for example recombinant antibodies that can be used in a pharmaceutical preparation or as a diagnostic tool. In one embodiment, the invention provides a method for obtaining a cell which expresses two or more proteins comprising providing said cell with two or more protein expression units encoding said two or more proteins, characterised in that at least two of said protein expression units comprise at least one STAR sequence wherin said STAR sequence protects said transgenes from the effect of gene silencing.

La présente invention concerne le domaine de la biochimie, de la biologie moléculaire, de la pharmacologie et du diagnostic. Plus particulièrement, cette invention concerne la production de protéines dans une cellule hôte, et plus précisément, encore, un procédé permettant d'améliorer l'expression d'au moins deux protéines dans une cellule (hôte). Ledit procédé convient à la production, par exemple, d'anticorps de recombinaison pouvant être utilisés dans une préparation pharmaceutique ou en tant qu'outil diagnostique. Un mode de réalisation concerne un procédé permettant d'obtenir une cellule exprimant au moins deux protéines, lequel procédé consiste à introduire dans la cellule au moins deux unités d'expression protéique codant pour lesdites protéines. Ces unités d'expression protéique présentent la caractéristique de contenir au moins une séquence STAR.

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