A method for stimulating root or shoot initiation in plant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 16/40 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2388346

The invention relates to the field of regeneration of cells and the vegetative propagation of (micro)-organisms or specific parts such as tissues or organs thereof, for example of those cells grown in tissue or organ culture, and more in particular to the seedless propagation of plants. The invention provides a culture method for propagation of a plant from plant starting material wherein during regeneration of said starting material, especially in the phase of the development of the shoot-root body plan, root or shoot initiation is stimulated by a recombinant gene product or functional fragment thereof, for example derived from a gene involved in the regulation of plant development allowing reducing or omitting exogenous phytohormone addition to said culture.

La présente invention concerne le domaine de la régénération de cellules et la propagation végétative de (micro)-organismes ou de parties spécifiques de ceux-ci, telles que des tissus ou des organes, par exemple des cellules cultivées dans des cultures de tissus ou d'organes, et plus particulièrement, la propagation de plantes apyrènes. L'invention concerne également un procédé de culture pour la propagation d'une plante à partir de matière générant une plante. Durant la régénération de cette matière de départ, surtout durant la phase de développement de racines et de pousses verticales, la génération des racines et des pousses est stimulée par un produit génique recombinant ou un fragment fonctionnel de celui-ci provenant, par exemple, d'un gène intervenant dans la régulation du développement de la plante, réduisant ainsi ou éliminant complètement l'addition de phytohormone exogène à ladite culture.

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