A method for sustainable transgene transcription

Details A method for sustainable transgene transcription A method for sustainable transgene transcription

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01N 25/00 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2685764

The present invention relates to constructs and methods for improving expression of transgenes in plants, animals and humans by including introns in the transcript to limit the exon sizes to less than about 60 bp. In the occurrence of dsRNA formation, methylation of the nuclear transgene construct DNA sequences and transcriptional silencing is not induced.

L'invention porte sur des gènes hybrides et des méthodes améliorant l'expression de transgènes dans des plantes, des animaux et chez l'homme en incluant des introns dans les transcrits pour limiter la taille des exons à moins d'environ 60bp, En cas de formation d'ARNds, la méthylation des séquences d'ADN des gènes hybrides des transgènes nucléaires et des silences transcriptionnels n'est pas induite..

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