C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2246238
A method is provided for the replication and detection of a specific nucleic acid target using a detection probe. The probe is present throughout the amplification reaction but does not participate in the reaction in that it is not extended. The probe contains sequence complementary to the replicated nucleic acid analyte for capture of the analyte by hybridization. Additionally, the probe or analyte contains at least one reactive ligand to permit immobilization or reporting of the probe/analyte hybrid.
L'invention porte sur un procédé de réplication et de détection d'une cible spécifique d'acide nucléique recourant à une sonde détectrice. Ladite sonde est présente lors de la totalité de la réaction d'amplification mais ne participe pas à la réaction en ce qu'elle n'est pas étendue. La sonde contient des séquences complémentaires de l'analyte d'acide nucléique répliqué pour capturer ledit analyte par hybridation. La sonde ou l'analyte peuvent en outre contenir au moins un ligand négatif permettant l'immobilisation ou le report de l'hybride sonde/analyte.
Ebersole Richard C.
Fitzpatrick-Mcelligott Sandra
Hendrickson Edwin R.
Perry Michael P.
Bennett Jones Llp
E.i. Du Pont de Nemours And Company
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