A method for the crystallization of human serum albumin

C - Chemistry – Metallurgy – 07 – K

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C07K 14/765 (2006.01) A61K 38/38 (2006.01) C07K 1/14 (2006.01)

Patent

CA 2506594

The present invention relates to the purification and production of human albumin from various sources through crystallization and repeated crystallization. Basic features of the invented process include providing specific reaction conditions and precipitating reagents to maximize albumin crystallization. Solubility diagrams are utilized as the basis for process control of the invented method. The current invention specifically controls phosphate concentration, pH and temperature to precisely guide crystallization kinetics and crystal yield.

L'invention concerne la purification et la production d'albumine humaine à partir de sources variées à l'aide de la cristallisation et la cristallisation répétée. Des caractéristiques de base du procédé de l'invention comprennent l'obtention des conditions de réaction spécifiques et la précipitation de réactifs, ceci permettant de maximiser la cristallisation de l'albumine. Les diagrammes de solubilité sont utilisés en tant que base pour le contrôle dudit procédé. L'invention permet de réguler de manière spécifique la concentration de phosphate, le pH et la température, de manière à diriger précisément la cinétique de cristallisation et le rendement du cristal.

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