C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) A61K 38/16 (2006.01) C12N 5/00 (2006.01) C12N 9/72 (2006.01) C12N 15/10 (2006.01) C12P 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/567 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2376131
The present invention discloses a method for the production of a mature recombinant protein into the culture medium of an eukaryotic cell line genetically transfected with a cloned precursor cDNA sequence, which comprises an incubation of said cell line in the cell culture medium wherein alkanoic acids, their derivatives or salts thereof have been added for a period of time of at least 24 hours. The process allows the secretion of recombinant proteins into the culture medium in their physiologically active (mature) form.
Arini Achille
Coppolecchia Raffaella
Herbst Detlev
Pagani Francesca Paola
Tognini Antonio
Cerbios-Pharma S.a.
Robic
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